Synthesis, scanning electron microscopy (SEM) and biocompatibility study of SLA 3D printable biopolymer hydrogel

József Bakó; Ferenc Tóth; Loránd Csámer; Lajos Daróczi; Csaba Hegedűs

doi:10.33891/FSZ.114.4.183-189

József Bakó University of Debrecen, Faculty of Dentistry, Department of Biomaterials and Prosthetic Dentistry https://orcid.org/0000-0003-1248-2943
Ferenc Tóth University of Debrecen, Faculty of Dentistry, Department of Biomaterials and Prosthetic Dentistry https://orcid.org/0000-0002-1170-6274
Loránd Csámer University of Debrecen, Faculty of Medicine, Department of Orthopaedic Surgery https://orcid.org/0000-0002-5023-3617
Lajos Daróczi University of Debrecen, Faculty of Science and Technology, Institute of Physics, Department of Solid State Physics https://orcid.org/0000-0002-9374-5225
Csaba Hegedűs University of Debrecen, Faculty of Dentistry, Department of Biomaterials and Prosthetic Dentistry https://orcid.org/0000-0003-4143-2507

DOI: https://doi.org/10.33891/FSZ.114.4.183-189

Keywords: 3D printing, Scaffold, Biopolymer, Hydrogel, Scanning electron microscopy

Abstract

Purpose: The demonstration of the production, SEM investigation and study of the biocompatibility of a biopolymerbased
3D printed hydrogel.
Materials and methods: Hydrogel samples with 1 and 2 mm thickness were planned by Ansys SpaceClaim (Ansys Inc,
USA) 3D modeling software. The biodegradable methacrylated-poly-γ-glutamic-acid (MPGA) polymer-based hydrogel
were produced by a stereolithographic (SLA) type Formlabs Form 2 (Formlabs Inc.) 3D printer. The surface and structure
of the hydrogels were studied by stereo and scanning electron microscopy (SEM) respectively. The biocompatibility
of the 3D printed samples was investigated by Alamar blue viability test using MG63 cells. The actual cells growing on
the surface of the samples were also examined by SEM.
Results: Our results showed that the MPGA based hydrogels were 3D printable by SLA technique. The printed
hydrogels are constructed by few hundred diameter nanofibers and web-like structures. The Alamar blue test showed
that, however, after 1 day of seeding, the numbers of the MG63 cells were significantly reduced at the hydrogel surface,
after another 3 days we could not detect any alteration in the cell number compared to that of the control. Additionally,
the SEM examination demonstrated the attachment of the cells to the surface of the hydrogel samples.
Conclusions: Our MPGA based polymer system were 3D printable by SLA technique. The prepared nanostructured
and biocompatible hydrogels might be promising vehicles for biologically active components in tissue engineering.

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Synthesis, scanning electron microscopy (SEM) and biocompatibility study of SLA 3D printable biopolymer hydrogel

Abstract

References

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