Taxonomic delimitation of Iris nyaradyana Prod. using molecular markers
Abstract
Iris nyaradyana Prod. named on the honour of the botanist Erazmus Gyula Nyárády was described in 1934, from rocks of the Upper Mureș/Maros River Valley, Romania. The taxonomic status of the species is still disputed; based on its morphology some authors identify it with I. aphylla, others with I. × germanica. Our study is the first to involve this taxon in molecular analysis, and aims to assess the relationship of the species based on molecular markers. Nuclear ITS and five chloroplast DNA markers (matk5–matk6, psbA5’R–matk8F, petL–psbE, psbM–trnD, trnL5’UAAF–trnFGAA) were used. Altogether 9 Iris species were compared and the sequence of 2 additional species (Crocus sp., Gladiolus sp.) were used as outgroups. One of the chloroplast markers, matk5–matk6 proved to be not variable and therefore was excluded from the analyses. Sequence analysis revealed that Iris nyaradyana is most closely related to I. × germanica, I. variegata, I. pallida and I. aphylla, but based on the molecular pattern, it is not identical to any of these species. Contrary to the opinion of some authors, molecular comparisons suggest that Iris nyaradyana is not a population of I. aphylla, although the high morphological variability of I. aphylla has been reported in previous publications. Templeton-Crandall-Sing (TCS) analysis of the chloroplast DNA sequences found the haplotypes of Iris nyaradyana to be similar to some haplotypes of I. × germanica and I. variegata. On the chloroplast phylogenetic tree, all three Iris nyaradyana individuals formed an independent clade with high support.
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Világháló-hivatkozás
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