Zinc monitoring on adults of Rana esculenta complex

  • Edina Simon Department of Ecology, University of Debrecen
  • Mihály Braun Department of Inorganic and Analytical Chemistry, University of Debrecen
  • Krisztián Szabó Biological Research Center of Hungarian Academic Institute of Genetics
  • Péter Molnár Department of Ecology, University of Debrecen
  • Béla Tóthmérész Department of Ecology, University of Debrecen
Keywords: amphibians, zinc accumulation, Túr, Miskafoki Holt-Tisza

Abstract

Concentration of zinc was examined on adults of Rana esculenta complex. Frogs were caught from two locations: the Túr river and an oxbow lake, called Miskafoki Holt-Tisza. The Túr river in North-East Hungary was contaminated with heavy metals, especially zinc, resulted in by mining activities. Zinc concentration was measured in the skin, thigh-bone, thigh-muscle, foot-bone, liver and frogs’ toes. There were no statistically significant difference in the zinc concentration in the skin, thigh-bone, thigh-muscle, footbone, and liver between the studied populations. The zinc concentration was significantly higher in the toes of the frogs in the Túr (mean ± SD: Túr 0.8 ± 0.13 µg/toe, Miskafoki Holt-Tisza 0.24 ± 0.04 µg/toe). Our results suggest that the level of zinc concentration is probably not harmful to the frog populations.

References

Beattie, R. C, Tyler-Loves, R. & Baxter, MJ. (1992): The effect of pH, aluminium concentration and temperature of embryonic development of the European common frog, Rana temporaria. - Zool. Lond. 228: 557-570.

Békési, I., Boga, T. L., Csiszár, L., Czédli, J., Horváth, Cs., Klingl, B., Nagy, G., Papanek, L., Pálfai, I., Pecze, J., Sajben, A., Sólymos, K. & Vörös, B. (2001): Magyarország holtágai. Közlekedési és Vízügyi Minisztérium, Budapest, pp. 106.

Bowness, J. M. & Morton, R. A. (1951): Distribution of copper and zinc in the eyes of fresh—water fishes and frogs. Occurrence of metals in melanin fractions from eye tissues. - Biochemical Journal. 51: 530-535.

Braun, M., Posta, J. & Löki, A. (1996): Kis térfogatú minták automatizált impulzus mintabeviteli módszerének kidolgozása atomabszorpciós és ICP atomemissziós spektrométerekhez. - XXXIX. Magyar Spektrokémiai Vándorgyűlés, Mosonmagyaróvár, 71—74.

Brown, J. R. & Chow, L. Y. (1977): Heavy metal concentrations in Ontario fish. - Bull. Environ. Contam. Toxicol. 17: 190-195.

Burger, J. & Snodgrass, J. (1998): Heavy metals in bullfrogs (Rana catesbeiana) tadpoles: effects of depuration before analysis. - Environ. Toxicol. Chem. 11: 2203-2209.

Coeurdassier, M., De Vaufleury, A. & Badot, P. M. (2003): Bioconcentration of cadmium and toxic effects on life-history traits of pond snails (Lymnaea palustris and Lymnaea stagnalis) in laboratory bioassays. - Arch. Environ. Contam. Toxicol. 45: 102-109.

Dely, O. Gy. (1967): Kétéltűek. - Fauna Hungáriáé, 20: 1-80.

Dowdy, S. & Wearden, S. (1983): Statistics for Research. - West Virginia University pp. 363-380.

Funk, W. C., Donelly, M. A. & Lips, K. R. (2005): Alternative views of amphibian toe-clipping. - Nature. 433:193.

Green, E. D. (2001): Toe-Clipping of frogs and toads. Amphibian Research and Monitoring Initative. - www.nwhc.usgs.gov/research/amph_dc/sop_ toeclip.html 2004.

Herkovits, J. & Perez-Coll, C.S. (1993): Stage-dependent suspectibility of Bufo arenarum embryos to cadmium. - Bull. Environ. Toxicol. 50: 608-611.

Herkovis, J., Cardellini, P, Pavanati, C. & Perez-Coll, C. S. (1997): Susceptibility of early life stages ofXenopus laevis to cadmium. - Environ. Toxicol. Chem. 16: 312-316.

James, S. M. & Little, E. E. (2003): The effects of chronic cadium exposure on american toad (Bufo americanus) tadpoles. -Environ. Toxicol. Chem. 22: 377-380.

Klerks, P. L. & Fraleigh P. C. (1997): Uptake to nickel and zinc by the zebra mussel Dreissena polymorpha. — Arch. Environ. Contam. Toxicol. 32: 191—197.

Kocsis Gáborné (szerk.) (1996): Szervetlen mikroszennyezők okozta terhelések felmérése a Túr folyó magyarországi szakaszán és a Túr belvízrendszeren. A vízi élőlény együttesekben történő nehézfém akkumulációk vizsgálata, különös tekintettel a Szatmár-Bereg Tájvédelmi körzet térségére. Kutatási téma zárójelentése, Nyíregyháza, pp. 1-185.

Loumbourdis, N. S. (1997): Heavy metal contamination in a lizard, Agama stellio stellio, compared in urban, high altitude and agricultural, low altitude areas of North Greece. - Bull. Environ. Contam. Toxicol. 58: 945-952.

May, R. M. (2004): Ethics and amphibians. - Nature. 431:404.

Muyssen, B. T. A. & Janssen, C. R. (2002): Accumulation and regulation of zinc Daphnia magna: links with homeostasis and toxicity. - Arch. Environ. Contam. Toxicol. 43: 492-496.

Naab, F., Volcomirsky, M., Burlón, A., Caraballo, M. E., Debray, M., Kesque J. M., Kreiner, A J., Ozafrán, M. J., Schuff, J. A., Stoliar, R, Vázquez, M. E., Davidson, J. & Fonovich de Schroeder, T. M. (2001): Metabolic alterations without metal accumulation in the ovary of adult Bufo arenarum females, observed after long-term exposure to Zn, followed by toxicity to embryos. - Arch. Environ. Contam. Toxicol. 41: 201-207.

Parris, K. M. & McCarthy, M. A.(2001): Identifying effects of toe-clipping on anuran return rates: the importance of statistical power. - Amphibia-Reptilia 22: 275—289.

Perez-Coll, C. S. & Herkovits, J. (1990): Stage-dependent suspectibility to lead inBufo arenarum embryos. - Env. Poll. 63: 239-245.

Perez-Coll, C. S. & Herkovits, J. (1996): Stage-dependent uptake of cadmium by Bufo arenarum embryos. - Bull. Environ. Contam. Toxicol. 56:663-669.

Puky, M. & Oertel, N. (1987): Schwermetallakkumulation in fröschen, die in mit donauwasser gespeisten fischteichen leben. -26. Arbeitstagung der IAD, Passau/Deutschland, Wissenschaftliche Kurzreferate pp. 56-60.

Puky, M. & Fodor, A. (2002): Occurrence of amphibian deformities along the Hungarian section of the river Danube, Tisza and Ipoly. - Internat. Assoc. Danube Res. 34: 845-852.

Smit, C. E., Schouten, A. J., Van den Brink, P. J., Esbroek, M. L. P. & Posthuma L. (2002): Effects of zinc contamination on a natural Nematoda community in outdoor soil mesocosms. - Arch. Environ. Contam. Toxicol. 42: 205-216.

Spurgeon, D. J. & Hopkin, S. P. (1999): Tolerance to zinc in populations of the earthworm Lumbricus rubellus from uncontaminated and metal-contaminated ecosystems. Arch. Environ. Contam. Toxicol. 37: 332-337.

Published
2007-12-31
Section
Scientific Research