Bioanalytics, lipidomics and metabolomics application of one- and twodimensional LC-MS techniques

Abstract

One of the most common coupled techniques in bioanalysis is liquid chromatography coupled mass spectrometry (LC-MS). Nowadays it is widespread technique in the fields of drug research, forensic science, food industry, biomedical research (proteomics, metabolomics, biomarker research) medical diagnostics or environmental protection as well. One-dimensional liquid chromatography (LC) is one of the most significant widespread separation techniques for the study of complex biological matrices. Offline and online two-dimensional liquid chromatography (2D-LC) have been shown to be powerful tools to resolve the coelution of the high number of analytes, in particular, in the field of proteomics, metabolomics and lipidomics. The main goal of my doctoral dissertation was to develop and apply one- and online two-dimensional LC-MS methods to answer biomedical questions in the bioanalytical approach.