Comparison of two freezing techniques and two seminal extenders in the cryopreservation of goat sperm
DOI:
https://doi.org/10.59913/dagr.2026.21976Keywords:
cryopreservation, goat, semen, Triladyl, OptixcellAbstract
The aim of this study was to compare the effect of two freezing techniques (programmable and manual) and two seminal extenders (Triladyl® and OptiXcell®) on the quality of cryopreserved goat sperm after thawing. The experiment involved ejaculates from goats of the Slovak White Shorthair Goat (n = 3) and Slovak Brown Shorthair Goat (n = 2), collected during the natural reproductive period of goats. After collection, the sperm were diluted, equilibrated for two hours at 4 °C and then frozen using either programmable or manual technique. After thawing, the progressive sperm motility was evaluated.
The results showed that the cryopreservation process led to a decrease in sperm motility compared to fresh samples. However, the use of the OptiXcell® extender ensured significantly higher progressive sperm motility after thawing compared to the Triladyl® extender (65.38% vs. 40.06%; p < 0.05) for both freezing techniques. On the contrary, no statistically significant differences were found between programmable and manual freezing, indicating their comparable effectiveness (36.85% vs. 40.06% and 57.13% vs. 65.38%, respectively).
Based on the results obtained, it can be concluded that the type of extender used has a more significant impact on sperm quality after thawing than the freezing technique itself. Manual freezing has proven to be a suitable alternative to programmable freezing, especially under conditions with no access to automated equipment, which may have significant practical benefits for the preservation of goat genetic resources in gene banks.
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